Localisation of proline oxidase and Delta-pyrroline-5-carboxylic acid dehydrogenase in rat liver.

The enzymes of prolinsglutamate oxidation and the preparation of their substrates have been described by Strecker and coworkers. [ 1,3] . The presence of proline oxidase in rat liver mitochondria was described by Johnson and Strecker [2]. A rough localisation of proline oxidase and A-pyrroline&carboxylic acid dehydrogenase in locust flight muscle has been attempted by Brosemer and Veerabhadrappa [4]. Lang and Lang [lo] reported proline oxidase to be bound to insoluble particles in rat liver cells. This communication describes the localisation of both enzymes in rat liver by the methods of fractionated extraction [ 5,6] and an improved method of preparing inner and outer membranes of rat liver mitochondria.